Cargando…

Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells

Bufalin has been shown to induce cancer cell death through apoptotic pathways. However, the molecular mechanisms are not well understood. In this study, we used the confocal fluorescence microscopy (CFM) to monitor the spatio-temporal dynamics of reactive oxygen species (ROS) production, Bax translo...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Lei, Chen, Tongsheng, Wang, Xiaoping, Chen, Yun, Wei, Xunbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136247/
https://www.ncbi.nlm.nih.gov/pubmed/19592481
http://dx.doi.org/10.1093/ecam/nep082
_version_ 1782208189844946944
author Sun, Lei
Chen, Tongsheng
Wang, Xiaoping
Chen, Yun
Wei, Xunbin
author_facet Sun, Lei
Chen, Tongsheng
Wang, Xiaoping
Chen, Yun
Wei, Xunbin
author_sort Sun, Lei
collection PubMed
description Bufalin has been shown to induce cancer cell death through apoptotic pathways. However, the molecular mechanisms are not well understood. In this study, we used the confocal fluorescence microscopy (CFM) to monitor the spatio-temporal dynamics of reactive oxygen species (ROS) production, Bax translocation and caspase-3 activation during bufalin-induced apoptosis in living human lung adenocarcinoma (ASTC-a-1) cells. Bufalin induced ROS production and apoptotic cell death, demonstrated by Hoechst 33258 staining as well as flow cytometry analysis. Bax redistributed from cytosol to mitochondria from 12 to 48 h after bufalin treatment in living cells expressed with green fluorescent protein Bax. Treatment with the antioxidant N-acetyl-cysteine (NAC), a ROS scavenger, inhibited ROS generation and Bax translocation and led to a significant protection against bufalin-induced apoptosis. Our results also revealed that bufalin induced a prominent increase of caspase-3 activation blocked potently by NAC. Taken together, bufalin induced ROS-mediated Bax translocation, mitochondrial permeability transition and caspase-3 activation, implying that bufalin induced apoptosis via ROS-dependent mitochondrial death pathway in ASTC-a-1 cells.
format Online
Article
Text
id pubmed-3136247
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-31362472011-07-26 Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells Sun, Lei Chen, Tongsheng Wang, Xiaoping Chen, Yun Wei, Xunbin Evid Based Complement Alternat Med Original Article Bufalin has been shown to induce cancer cell death through apoptotic pathways. However, the molecular mechanisms are not well understood. In this study, we used the confocal fluorescence microscopy (CFM) to monitor the spatio-temporal dynamics of reactive oxygen species (ROS) production, Bax translocation and caspase-3 activation during bufalin-induced apoptosis in living human lung adenocarcinoma (ASTC-a-1) cells. Bufalin induced ROS production and apoptotic cell death, demonstrated by Hoechst 33258 staining as well as flow cytometry analysis. Bax redistributed from cytosol to mitochondria from 12 to 48 h after bufalin treatment in living cells expressed with green fluorescent protein Bax. Treatment with the antioxidant N-acetyl-cysteine (NAC), a ROS scavenger, inhibited ROS generation and Bax translocation and led to a significant protection against bufalin-induced apoptosis. Our results also revealed that bufalin induced a prominent increase of caspase-3 activation blocked potently by NAC. Taken together, bufalin induced ROS-mediated Bax translocation, mitochondrial permeability transition and caspase-3 activation, implying that bufalin induced apoptosis via ROS-dependent mitochondrial death pathway in ASTC-a-1 cells. Hindawi Publishing Corporation 2011 2011-06-18 /pmc/articles/PMC3136247/ /pubmed/19592481 http://dx.doi.org/10.1093/ecam/nep082 Text en Copyright © 2011 Lei Sun et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sun, Lei
Chen, Tongsheng
Wang, Xiaoping
Chen, Yun
Wei, Xunbin
Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title_full Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title_fullStr Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title_full_unstemmed Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title_short Bufalin Induces Reactive Oxygen Species Dependent Bax Translocation and Apoptosis in ASTC-a-1 Cells
title_sort bufalin induces reactive oxygen species dependent bax translocation and apoptosis in astc-a-1 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136247/
https://www.ncbi.nlm.nih.gov/pubmed/19592481
http://dx.doi.org/10.1093/ecam/nep082
work_keys_str_mv AT sunlei bufalininducesreactiveoxygenspeciesdependentbaxtranslocationandapoptosisinastca1cells
AT chentongsheng bufalininducesreactiveoxygenspeciesdependentbaxtranslocationandapoptosisinastca1cells
AT wangxiaoping bufalininducesreactiveoxygenspeciesdependentbaxtranslocationandapoptosisinastca1cells
AT chenyun bufalininducesreactiveoxygenspeciesdependentbaxtranslocationandapoptosisinastca1cells
AT weixunbin bufalininducesreactiveoxygenspeciesdependentbaxtranslocationandapoptosisinastca1cells