A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300

A gene encoding a putative DNA helicase from Staphylococcus aureus USA300 was cloned and expressed in Escherichia coli. The protein was purified to over 90% purity by chromatography. The purified enzyme, SauUSI, predominantly cleaves modified DNA containing 5mC and 5-hydroxymethylcytosine. Cleavage...

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Detalles Bibliográficos
Autores principales: Xu, Shuang-yong, Corvaglia, Anna R., Chan, Siu-Hong, Zheng, Yu, Linder, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141236/
https://www.ncbi.nlm.nih.gov/pubmed/21421560
http://dx.doi.org/10.1093/nar/gkr098

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141236/
https://www.ncbi.nlm.nih.gov/pubmed/21421560
http://dx.doi.org/10.1093/nar/gkr098

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