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Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryo...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141258/ https://www.ncbi.nlm.nih.gov/pubmed/21447562 http://dx.doi.org/10.1093/nar/gkr148 |
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author | Gantier, Michael P. McCoy, Claire E. Rusinova, Irina Saulep, Damien Wang, Die Xu, Dakang Irving, Aaron T. Behlke, Mark A. Hertzog, Paul J. Mackay, Fabienne Williams, Bryan R. G. |
author_facet | Gantier, Michael P. McCoy, Claire E. Rusinova, Irina Saulep, Damien Wang, Die Xu, Dakang Irving, Aaron T. Behlke, Mark A. Hertzog, Paul J. Mackay, Fabienne Williams, Bryan R. G. |
author_sort | Gantier, Michael P. |
collection | PubMed |
description | Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10× more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function. |
format | Online Article Text |
id | pubmed-3141258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31412582011-07-22 Analysis of microRNA turnover in mammalian cells following Dicer1 ablation Gantier, Michael P. McCoy, Claire E. Rusinova, Irina Saulep, Damien Wang, Die Xu, Dakang Irving, Aaron T. Behlke, Mark A. Hertzog, Paul J. Mackay, Fabienne Williams, Bryan R. G. Nucleic Acids Res RNA Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10× more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function. Oxford University Press 2011-07 2011-03-28 /pmc/articles/PMC3141258/ /pubmed/21447562 http://dx.doi.org/10.1093/nar/gkr148 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Gantier, Michael P. McCoy, Claire E. Rusinova, Irina Saulep, Damien Wang, Die Xu, Dakang Irving, Aaron T. Behlke, Mark A. Hertzog, Paul J. Mackay, Fabienne Williams, Bryan R. G. Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title_full | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title_fullStr | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title_full_unstemmed | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title_short | Analysis of microRNA turnover in mammalian cells following Dicer1 ablation |
title_sort | analysis of microrna turnover in mammalian cells following dicer1 ablation |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141258/ https://www.ncbi.nlm.nih.gov/pubmed/21447562 http://dx.doi.org/10.1093/nar/gkr148 |
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