Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA template is attached to a functionalized glass coverslip and replicated extensively after introduction of replication proteins and nucleotides (Figur...

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Detalles Bibliográficos
Autores principales: Tanner, Nathan A., Loparo, Joseph J., van Oijen, Antoine M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2009
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142874/
https://www.ncbi.nlm.nih.gov/pubmed/19820685
http://dx.doi.org/10.3791/1529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142874/
https://www.ncbi.nlm.nih.gov/pubmed/19820685
http://dx.doi.org/10.3791/1529

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