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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

We describe a simple fluorescence microscopy-based real-time method for observing DNA replication at the single-molecule level. A circular, forked DNA template is attached to a functionalized glass coverslip and replicated extensively after introduction of replication proteins and nucleotides (Figur...

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Detalles Bibliográficos
Autores principales:	Tanner, Nathan A., Loparo, Joseph J., van Oijen, Antoine M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2009
Materias:	Cellular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142874/ https://www.ncbi.nlm.nih.gov/pubmed/19820685 http://dx.doi.org/10.3791/1529

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