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Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144238/ https://www.ncbi.nlm.nih.gov/pubmed/21818365 http://dx.doi.org/10.1371/journal.pone.0022678 |