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Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...

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Detalles Bibliográficos
Autores principales: Annibale, Paolo, Vanni, Stefano, Scarselli, Marco, Rothlisberger, Ursula, Radenovic, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144238/
https://www.ncbi.nlm.nih.gov/pubmed/21818365
http://dx.doi.org/10.1371/journal.pone.0022678