Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...

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Detalles Bibliográficos
Autores principales: Annibale, Paolo, Vanni, Stefano, Scarselli, Marco, Rothlisberger, Ursula, Radenovic, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144238/
https://www.ncbi.nlm.nih.gov/pubmed/21818365
http://dx.doi.org/10.1371/journal.pone.0022678
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author Annibale, Paolo
Vanni, Stefano
Scarselli, Marco
Rothlisberger, Ursula
Radenovic, Aleksandra
author_facet Annibale, Paolo
Vanni, Stefano
Scarselli, Marco
Rothlisberger, Ursula
Radenovic, Aleksandra
author_sort Annibale, Paolo
collection PubMed
description In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
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spelling pubmed-31442382011-08-04 Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking Annibale, Paolo Vanni, Stefano Scarselli, Marco Rothlisberger, Ursula Radenovic, Aleksandra PLoS One Research Article In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells. Public Library of Science 2011-07-26 /pmc/articles/PMC3144238/ /pubmed/21818365 http://dx.doi.org/10.1371/journal.pone.0022678 Text en Annibale et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Annibale, Paolo
Vanni, Stefano
Scarselli, Marco
Rothlisberger, Ursula
Radenovic, Aleksandra
Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title_full Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title_fullStr Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title_full_unstemmed Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title_short Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking
title_sort quantitative photo activated localization microscopy: unraveling the effects of photoblinking
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144238/
https://www.ncbi.nlm.nih.gov/pubmed/21818365
http://dx.doi.org/10.1371/journal.pone.0022678
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