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Pyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens
DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144868/ https://www.ncbi.nlm.nih.gov/pubmed/21818256 http://dx.doi.org/10.1371/journal.pone.0021252 |