Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101

The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5′-gtgtcaagaatgtttgc-3′ and BS168R: 3′-gttttgttaaaagatga-5′ primers were used to amplify the amylase gene using the following cycle in error-prone PCR method: 94°C for 30 s, 40°C...

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Detalles Bibliográficos
Autores principales: Rabbani, Mohammed, MirMohammad Sadeghi, Hamid, Moazen, Fatemeh, Rahimi, Mehran, Salehi, Golnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145346/
https://www.ncbi.nlm.nih.gov/pubmed/21808739
http://dx.doi.org/10.4061/2011/305956

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145346/
https://www.ncbi.nlm.nih.gov/pubmed/21808739
http://dx.doi.org/10.4061/2011/305956

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