Cargando…
Cloning and Expression of Randomly Mutated Bacillus subtilis α-Amylase Genes in HB101
The aim of this study was to isolate and express the randomly mutated α-amylase gene from B. subtilis strain 168. BS168F: 5′-gtgtcaagaatgtttgc-3′ and BS168R: 3′-gttttgttaaaagatga-5′ primers were used to amplify the amylase gene using the following cycle in error-prone PCR method: 94°C for 30 s, 40°C...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
SAGE-Hindawi Access to Research
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145346/ https://www.ncbi.nlm.nih.gov/pubmed/21808739 http://dx.doi.org/10.4061/2011/305956 |