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Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons

BACKGROUND: Bacterial Artificial Chromosomes (BACs) have been widely used as transgenes in vertebrate model systems such as mice and zebrafish, for a variety of studies. BAC transgenesis has been a powerful tool to study the function of the genome, and gene regulation by distal cis-regulatory elemen...

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Detalles Bibliográficos
Autores principales: Shakes, Leighcraft A, Abe, Gembu, Eltayeb, Mugtaba A, Wolf, Hope M, Kawakami, Koichi, Chatterjee, Pradeep K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146455/
https://www.ncbi.nlm.nih.gov/pubmed/21736732
http://dx.doi.org/10.1186/1471-2164-12-351