Checkpoints in Adenoviral Production: Cross-Contamination and E1A

Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact c...

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Detalles Bibliográficos
Autores principales: Haeussler, Dagmar J., Evangelista, Alicia M., Burgoyne, Joseph R., Cohen, Richard A., Bachschmid, Markus M., Pimental, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149638/
https://www.ncbi.nlm.nih.gov/pubmed/21826235
http://dx.doi.org/10.1371/journal.pone.0023160
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author Haeussler, Dagmar J.
Evangelista, Alicia M.
Burgoyne, Joseph R.
Cohen, Richard A.
Bachschmid, Markus M.
Pimental, David R.
author_facet Haeussler, Dagmar J.
Evangelista, Alicia M.
Burgoyne, Joseph R.
Cohen, Richard A.
Bachschmid, Markus M.
Pimental, David R.
author_sort Haeussler, Dagmar J.
collection PubMed
description Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.
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spelling pubmed-31496382011-08-08 Checkpoints in Adenoviral Production: Cross-Contamination and E1A Haeussler, Dagmar J. Evangelista, Alicia M. Burgoyne, Joseph R. Cohen, Richard A. Bachschmid, Markus M. Pimental, David R. PLoS One Research Article Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA. Public Library of Science 2011-08-03 /pmc/articles/PMC3149638/ /pubmed/21826235 http://dx.doi.org/10.1371/journal.pone.0023160 Text en Haeussler et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Haeussler, Dagmar J.
Evangelista, Alicia M.
Burgoyne, Joseph R.
Cohen, Richard A.
Bachschmid, Markus M.
Pimental, David R.
Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title_full Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title_fullStr Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title_full_unstemmed Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title_short Checkpoints in Adenoviral Production: Cross-Contamination and E1A
title_sort checkpoints in adenoviral production: cross-contamination and e1a
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149638/
https://www.ncbi.nlm.nih.gov/pubmed/21826235
http://dx.doi.org/10.1371/journal.pone.0023160
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