Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

We present a method of targeted DNA sequence retrieval from DNA sources which are heavily degraded and contaminated with microbial DNA, as is typical of ancient bones. The method greatly reduces sample destruction and sequencing demands relative to direct PCR or shotgun sequencing approaches. We use...

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Detalles Bibliográficos
Autores principales: Briggs, Adrian W., Good, Jeffrey M., Green, Richard E., Krause, Johannes, Maricic, Tomislav, Stenzel, Udo, Pääbo, Svante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2009
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150061/
https://www.ncbi.nlm.nih.gov/pubmed/19730410
http://dx.doi.org/10.3791/1573

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150061/
https://www.ncbi.nlm.nih.gov/pubmed/19730410
http://dx.doi.org/10.3791/1573

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