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Freezing and Thawing Human Embryonic Stem Cells
Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells from a frozen stock have become important procedures performed in routine hES cell culture. Since hES cells are very sensitive to the stresses of f...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MyJove Corporation
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152242/ https://www.ncbi.nlm.nih.gov/pubmed/20035272 http://dx.doi.org/10.3791/1555 |