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Confocal Fluorescence Anisotropy and FRAP Imaging of α-Synuclein Amyloid Aggregates in Living Cells

We assessed the intracellular association states of the Parkinson's disease related protein α-synuclein (AS) in living cells by transfection with a functional recombinant mutant protein (AS-C4) bearing a tetracysteine tag binding the fluorogenic biarsenical ligands FlAsH and ReAsH, The aggregat...

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Detalles Bibliográficos
Autores principales: Roberti, M. Julia, Jovin, Thomas M., Jares-Erijman, Elizabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3152574/
https://www.ncbi.nlm.nih.gov/pubmed/21858077
http://dx.doi.org/10.1371/journal.pone.0023338