An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones

BACKGROUND: Gene targeting by homologous recombination using recombinant adeno-associated virus (rAAV) is becoming a useful tool for basic research and therapeutic applications due to the remarkably high targeting frequency of rAAV virus vectors. However, the screening for the pure gene-targeted and...

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Detalles Bibliográficos
Autores principales: Luo, Yonglun, Bolund, Lars, Sørensen, Charlotte B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154164/
https://www.ncbi.nlm.nih.gov/pubmed/21777457
http://dx.doi.org/10.1186/1756-0500-4-246

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3154164/
https://www.ncbi.nlm.nih.gov/pubmed/21777457
http://dx.doi.org/10.1186/1756-0500-4-246

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