Target-selective joint polymerase chain reaction: A robust and rapid method for high-throughput production of recombinant monoclonal antibodies from single cells

BACKGROUND: During the development of a therapeutic antibody, large numbers of monoclonal antibodies are required to screen for those that are best suited for the desired activity. Although the single cell-based immunoglobulin variable gene cloning technique is a powerful tool, the current methods r...

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Detalles Bibliográficos
Autores principales: Yoshioka, Megumi, Kurosawa, Nobuyuki, Isobe, Masaharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155496/
https://www.ncbi.nlm.nih.gov/pubmed/21774833
http://dx.doi.org/10.1186/1472-6750-11-75

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155496/
https://www.ncbi.nlm.nih.gov/pubmed/21774833
http://dx.doi.org/10.1186/1472-6750-11-75

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