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A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles

Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format. In addition, alkali minipreps usually require additional column-based purification steps and cannot isolate other extra-chromosoma...

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Detalles Bibliográficos
Autores principales: Lezin, George, Kosaka, Yasuhiro, Yost, H. Joseph, Kuehn, Michael R., Brunelli, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156146/
https://www.ncbi.nlm.nih.gov/pubmed/21858126
http://dx.doi.org/10.1371/journal.pone.0023457