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A One-Step Miniprep for the Isolation of Plasmid DNA and Lambda Phage Particles
Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format. In addition, alkali minipreps usually require additional column-based purification steps and cannot isolate other extra-chromosoma...
Autores principales: | Lezin, George, Kosaka, Yasuhiro, Yost, H. Joseph, Kuehn, Michael R., Brunelli, Luca |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156146/ https://www.ncbi.nlm.nih.gov/pubmed/21858126 http://dx.doi.org/10.1371/journal.pone.0023457 |
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