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Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats

BACKGROUND: Rapeseed is an emerging and promising source of dietary protein for human nutrition and health. We previously found that rapeseed protein displayed atypical nutritional properties in humans, characterized by low bioavailability and a high postprandial biological value. The objective of t...

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Autores principales: Boutry , Claire, Fouillet, Hélène, Mariotti, François, Blachier, François, Tomé, Daniel, Bos, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156721/
https://www.ncbi.nlm.nih.gov/pubmed/21787407
http://dx.doi.org/10.1186/1743-7075-8-52
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author Boutry , Claire
Fouillet, Hélène
Mariotti, François
Blachier, François
Tomé, Daniel
Bos, Cécile
author_facet Boutry , Claire
Fouillet, Hélène
Mariotti, François
Blachier, François
Tomé, Daniel
Bos, Cécile
author_sort Boutry , Claire
collection PubMed
description BACKGROUND: Rapeseed is an emerging and promising source of dietary protein for human nutrition and health. We previously found that rapeseed protein displayed atypical nutritional properties in humans, characterized by low bioavailability and a high postprandial biological value. The objective of the present study was to investigate the metabolic fate of rapeseed protein isolate (RPI) and its effect on protein fractional synthesis rates (FSR) in various tissues when compared to a milk protein isolate (MPI). METHODS: Rats (n = 48) were given a RPI or MPI meal, either for the first time or after 2-week adaptation to a MPI or RPI-based diet. They were divided in two groups for measuring the fed-state tissue FSR 2 h after the meal (using a flooding dose of (13)C-valine) and the dietary N postprandial distribution at 5 h (using (15)N-labeled meals). RESULTS: RPI and MPI led to similar FSR and dietary nitrogen (N) losses (ileal and deamination losses of 4% and 12% of the meal, respectively). By contrast, the dietary N incorporation was significantly higher in the intestinal mucosa and liver (+36% and +16%, respectively) and lower in skin (-24%) after RPI than MPI. CONCLUSIONS: Although RPI and MPI led to the same overall level of postprandial dietary N retention in rats (in line with our findings in humans), this global response conceals marked qualitative differences at the tissue level regarding dietary N accretion. The fact that FSR did not however differed between groups suggest a differential modulation of proteolysis after RPI or MPI ingestion, or other mechanisms that warrant further study.
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spelling pubmed-31567212011-08-17 Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats Boutry , Claire Fouillet, Hélène Mariotti, François Blachier, François Tomé, Daniel Bos, Cécile Nutr Metab (Lond) Research BACKGROUND: Rapeseed is an emerging and promising source of dietary protein for human nutrition and health. We previously found that rapeseed protein displayed atypical nutritional properties in humans, characterized by low bioavailability and a high postprandial biological value. The objective of the present study was to investigate the metabolic fate of rapeseed protein isolate (RPI) and its effect on protein fractional synthesis rates (FSR) in various tissues when compared to a milk protein isolate (MPI). METHODS: Rats (n = 48) were given a RPI or MPI meal, either for the first time or after 2-week adaptation to a MPI or RPI-based diet. They were divided in two groups for measuring the fed-state tissue FSR 2 h after the meal (using a flooding dose of (13)C-valine) and the dietary N postprandial distribution at 5 h (using (15)N-labeled meals). RESULTS: RPI and MPI led to similar FSR and dietary nitrogen (N) losses (ileal and deamination losses of 4% and 12% of the meal, respectively). By contrast, the dietary N incorporation was significantly higher in the intestinal mucosa and liver (+36% and +16%, respectively) and lower in skin (-24%) after RPI than MPI. CONCLUSIONS: Although RPI and MPI led to the same overall level of postprandial dietary N retention in rats (in line with our findings in humans), this global response conceals marked qualitative differences at the tissue level regarding dietary N accretion. The fact that FSR did not however differed between groups suggest a differential modulation of proteolysis after RPI or MPI ingestion, or other mechanisms that warrant further study. BioMed Central 2011-07-25 /pmc/articles/PMC3156721/ /pubmed/21787407 http://dx.doi.org/10.1186/1743-7075-8-52 Text en Copyright ©2011 Boutry et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Boutry , Claire
Fouillet, Hélène
Mariotti, François
Blachier, François
Tomé, Daniel
Bos, Cécile
Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title_full Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title_fullStr Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title_full_unstemmed Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title_short Rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
title_sort rapeseed and milk protein exhibit a similar overall nutritional value but marked difference in postprandial regional nitrogen utilization in rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156721/
https://www.ncbi.nlm.nih.gov/pubmed/21787407
http://dx.doi.org/10.1186/1743-7075-8-52
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