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A rapid and easy method for the DNA extraction from Cryptococcus neoformans

DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported meth...

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Detalles Bibliográficos
Autores principales: Mseddi, Fatma, Jarboui, Mohammed Ali, Sellami, Amira, Sellami, Hayet, Ayadi, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156736/
https://www.ncbi.nlm.nih.gov/pubmed/21777412
http://dx.doi.org/10.1186/1480-9222-13-5