A rapid and easy method for the DNA extraction from Cryptococcus neoformans

DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported meth...

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Detalles Bibliográficos
Autores principales: Mseddi, Fatma, Jarboui, Mohammed Ali, Sellami, Amira, Sellami, Hayet, Ayadi, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156736/
https://www.ncbi.nlm.nih.gov/pubmed/21777412
http://dx.doi.org/10.1186/1480-9222-13-5
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author Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
author_facet Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
author_sort Mseddi, Fatma
collection PubMed
description DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.
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spelling pubmed-31567362011-08-17 A rapid and easy method for the DNA extraction from Cryptococcus neoformans Mseddi, Fatma Jarboui, Mohammed Ali Sellami, Amira Sellami, Hayet Ayadi, Ali Biol Proced Online Research DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods. BioMed Central 2011-07-21 /pmc/articles/PMC3156736/ /pubmed/21777412 http://dx.doi.org/10.1186/1480-9222-13-5 Text en Copyright ©2011 Mseddi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Mseddi, Fatma
Jarboui, Mohammed Ali
Sellami, Amira
Sellami, Hayet
Ayadi, Ali
A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_full A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_fullStr A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_full_unstemmed A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_short A rapid and easy method for the DNA extraction from Cryptococcus neoformans
title_sort rapid and easy method for the dna extraction from cryptococcus neoformans
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156736/
https://www.ncbi.nlm.nih.gov/pubmed/21777412
http://dx.doi.org/10.1186/1480-9222-13-5
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