The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade

The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associa...

Descripción completa

Detalles Bibliográficos
Autores principales: Bonomelli, Camille, Doores, Katie J., Dunlop, D. Cameron, Thaney, Victoria, Dwek, Raymond A., Burton, Dennis R., Crispin, Max, Scanlan, Christopher N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156772/
https://www.ncbi.nlm.nih.gov/pubmed/21858152
http://dx.doi.org/10.1371/journal.pone.0023521
_version_ 1782210235390230528
author Bonomelli, Camille
Doores, Katie J.
Dunlop, D. Cameron
Thaney, Victoria
Dwek, Raymond A.
Burton, Dennis R.
Crispin, Max
Scanlan, Christopher N.
author_facet Bonomelli, Camille
Doores, Katie J.
Dunlop, D. Cameron
Thaney, Victoria
Dwek, Raymond A.
Burton, Dennis R.
Crispin, Max
Scanlan, Christopher N.
author_sort Bonomelli, Camille
collection PubMed
description The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62–79%) of these glycans relative to recombinant, monomeric gp120 (∼30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (∼98%), compared to gp120 derived from a single-plasmid viral system using the HIV(LAI) backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.
format Online
Article
Text
id pubmed-3156772
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31567722011-08-19 The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade Bonomelli, Camille Doores, Katie J. Dunlop, D. Cameron Thaney, Victoria Dwek, Raymond A. Burton, Dennis R. Crispin, Max Scanlan, Christopher N. PLoS One Research Article The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62–79%) of these glycans relative to recombinant, monomeric gp120 (∼30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (∼98%), compared to gp120 derived from a single-plasmid viral system using the HIV(LAI) backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120. Public Library of Science 2011-08-16 /pmc/articles/PMC3156772/ /pubmed/21858152 http://dx.doi.org/10.1371/journal.pone.0023521 Text en Bonomelli et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bonomelli, Camille
Doores, Katie J.
Dunlop, D. Cameron
Thaney, Victoria
Dwek, Raymond A.
Burton, Dennis R.
Crispin, Max
Scanlan, Christopher N.
The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title_full The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title_fullStr The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title_full_unstemmed The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title_short The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade
title_sort glycan shield of hiv is predominantly oligomannose independently of production system or viral clade
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3156772/
https://www.ncbi.nlm.nih.gov/pubmed/21858152
http://dx.doi.org/10.1371/journal.pone.0023521
work_keys_str_mv AT bonomellicamille theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dooreskatiej theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dunlopdcameron theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT thaneyvictoria theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dwekraymonda theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT burtondennisr theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT crispinmax theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT scanlanchristophern theglycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT bonomellicamille glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dooreskatiej glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dunlopdcameron glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT thaneyvictoria glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT dwekraymonda glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT burtondennisr glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT crispinmax glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade
AT scanlanchristophern glycanshieldofhivispredominantlyoligomannoseindependentlyofproductionsystemorviralclade

Ejemplares similares