A protocol for enumeration of aquatic viruses by epifluorescence microscopy using Anodisc™ 13 membranes

BACKGROUND: Epifluorescence microscopy is a common method used to enumerate virus-like particles (VLP) from environmental samples and relies on the use of filter membranes with pore sizes < 0.02 μm; the most commonly used protocols employ 25 mm Anodisc™ membranes with a built-in support ring. Oth...

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Detalles Bibliográficos
Autores principales: Budinoff, Charles R, Loar, Star N, LeCleir, Gary R, Wilhelm, Steven W, Buchan, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157413/
https://www.ncbi.nlm.nih.gov/pubmed/21787406
http://dx.doi.org/10.1186/1471-2180-11-168

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3157413/
https://www.ncbi.nlm.nih.gov/pubmed/21787406
http://dx.doi.org/10.1186/1471-2180-11-168

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