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Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice
BACKGROUND: Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium ps...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158118/ https://www.ncbi.nlm.nih.gov/pubmed/21774825 http://dx.doi.org/10.1186/1756-0500-4-243 |
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author | Costa, Marcilia P McCulloch, John A Almeida, Síntia S Dorella, Fernanda A Fonseca, Cristina T Oliveira, Diana M Teixeira, Maria FS Laskowska, Ewa Lipinska, Barbara Meyer, Roberto Portela, Ricardo W Oliveira, Sérgio C Miyoshi, Anderson Azevedo, Vasco |
author_facet | Costa, Marcilia P McCulloch, John A Almeida, Síntia S Dorella, Fernanda A Fonseca, Cristina T Oliveira, Diana M Teixeira, Maria FS Laskowska, Ewa Lipinska, Barbara Meyer, Roberto Portela, Ricardo W Oliveira, Sérgio C Miyoshi, Anderson Azevedo, Vasco |
author_sort | Costa, Marcilia P |
collection | PubMed |
description | BACKGROUND: Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants. FINDINGS: To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60). CONCLUSION: This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity. |
format | Online Article Text |
id | pubmed-3158118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31581182011-08-19 Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice Costa, Marcilia P McCulloch, John A Almeida, Síntia S Dorella, Fernanda A Fonseca, Cristina T Oliveira, Diana M Teixeira, Maria FS Laskowska, Ewa Lipinska, Barbara Meyer, Roberto Portela, Ricardo W Oliveira, Sérgio C Miyoshi, Anderson Azevedo, Vasco BMC Res Notes Short Report BACKGROUND: Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants. FINDINGS: To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60). CONCLUSION: This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity. BioMed Central 2011-07-20 /pmc/articles/PMC3158118/ /pubmed/21774825 http://dx.doi.org/10.1186/1756-0500-4-243 Text en Copyright ©2011 Azevedo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Costa, Marcilia P McCulloch, John A Almeida, Síntia S Dorella, Fernanda A Fonseca, Cristina T Oliveira, Diana M Teixeira, Maria FS Laskowska, Ewa Lipinska, Barbara Meyer, Roberto Portela, Ricardo W Oliveira, Sérgio C Miyoshi, Anderson Azevedo, Vasco Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title_full | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title_fullStr | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title_full_unstemmed | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title_short | Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice |
title_sort | molecular characterization of the corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 dna vaccination in mice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158118/ https://www.ncbi.nlm.nih.gov/pubmed/21774825 http://dx.doi.org/10.1186/1756-0500-4-243 |
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