d-Xylulose kinase from Saccharomyces cerevisiae: Isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli

The Saccharomyces cerevisiae gene encoding xylulose kinase (XKS1) was over-expressed to an abundance of ⩾10% intracellular protein in Escherichia coli. Instability of XKS1, not pointed out in previous reports of the enzyme, prevented isolation of active enzyme in native or “tagged” form under a wide...

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Detalles Bibliográficos
Autores principales: Pival, Simone L., Birner-Gruenberger, Ruth, Krump, Corinna, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158326/
https://www.ncbi.nlm.nih.gov/pubmed/21664974
http://dx.doi.org/10.1016/j.pep.2011.05.018

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158326/
https://www.ncbi.nlm.nih.gov/pubmed/21664974
http://dx.doi.org/10.1016/j.pep.2011.05.018

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