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Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells

Mammalian germ cells undergo global reprogramming of DNA methylation during their development. Global DNA demethylation occurs around the time when the primordial germ cells colonize the embryonic gonads and this coincides with dynamic changes in chromatin composition. Global de novo DNA methylation...

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Autores principales: Abe, Masanobu, Tsai, Shirley Y., Jin, Seung-Gi, Pfeifer, Gerd P., Szabó, Piroska E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158789/
https://www.ncbi.nlm.nih.gov/pubmed/21886830
http://dx.doi.org/10.1371/journal.pone.0023848
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author Abe, Masanobu
Tsai, Shirley Y.
Jin, Seung-Gi
Pfeifer, Gerd P.
Szabó, Piroska E.
author_facet Abe, Masanobu
Tsai, Shirley Y.
Jin, Seung-Gi
Pfeifer, Gerd P.
Szabó, Piroska E.
author_sort Abe, Masanobu
collection PubMed
description Mammalian germ cells undergo global reprogramming of DNA methylation during their development. Global DNA demethylation occurs around the time when the primordial germ cells colonize the embryonic gonads and this coincides with dynamic changes in chromatin composition. Global de novo DNA methylation takes place with remarkably different dynamics between the two sexes, prospermatogonia attaining methylation during fetal stages and oocytes attaining methylation postnatally. Our hypothesis was that dynamic changes in chromatin composition may precede or accompany the wave of global DNA de novo methylation as well. We used immunocytochemistry to measure global DNA methylation and chromatin components in male and female mouse fetal germ cells compared to control somatic cells of the gonad. We found that global DNA methylation levels sharply increased in male germ cells at 17.5 days post coitum, but remained low in female germ cells at all fetal stages. Global changes in chromatin composition: i, preceded global DNA methylation in fetal germ cells; ii, sex specifically occurred in male but not in female germ cells; iii, affected active and repressive histone marks and iv, included histone tail and histone globular domain modifications. Our data suggest that dynamic changes of chromatin composition may provide a framework for the pattern of male-specific de novo DNA methylation in prospermatogonia.
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spelling pubmed-31587892011-08-30 Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells Abe, Masanobu Tsai, Shirley Y. Jin, Seung-Gi Pfeifer, Gerd P. Szabó, Piroska E. PLoS One Research Article Mammalian germ cells undergo global reprogramming of DNA methylation during their development. Global DNA demethylation occurs around the time when the primordial germ cells colonize the embryonic gonads and this coincides with dynamic changes in chromatin composition. Global de novo DNA methylation takes place with remarkably different dynamics between the two sexes, prospermatogonia attaining methylation during fetal stages and oocytes attaining methylation postnatally. Our hypothesis was that dynamic changes in chromatin composition may precede or accompany the wave of global DNA de novo methylation as well. We used immunocytochemistry to measure global DNA methylation and chromatin components in male and female mouse fetal germ cells compared to control somatic cells of the gonad. We found that global DNA methylation levels sharply increased in male germ cells at 17.5 days post coitum, but remained low in female germ cells at all fetal stages. Global changes in chromatin composition: i, preceded global DNA methylation in fetal germ cells; ii, sex specifically occurred in male but not in female germ cells; iii, affected active and repressive histone marks and iv, included histone tail and histone globular domain modifications. Our data suggest that dynamic changes of chromatin composition may provide a framework for the pattern of male-specific de novo DNA methylation in prospermatogonia. Public Library of Science 2011-08-19 /pmc/articles/PMC3158789/ /pubmed/21886830 http://dx.doi.org/10.1371/journal.pone.0023848 Text en Abe et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Abe, Masanobu
Tsai, Shirley Y.
Jin, Seung-Gi
Pfeifer, Gerd P.
Szabó, Piroska E.
Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title_full Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title_fullStr Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title_full_unstemmed Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title_short Sex-Specific Dynamics of Global Chromatin Changes in Fetal Mouse Germ Cells
title_sort sex-specific dynamics of global chromatin changes in fetal mouse germ cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158789/
https://www.ncbi.nlm.nih.gov/pubmed/21886830
http://dx.doi.org/10.1371/journal.pone.0023848
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