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Label-free optical imaging of membrane patches for atomic force microscopy
In atomic force microscopy (AFM), finding sparsely distributed regions of interest can be difficult and time-consuming. Typically, the tip is scanned until the desired object is located. This process can mechanically or chemically degrade the tip, as well as damage fragile biological samples. Protei...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158999/ https://www.ncbi.nlm.nih.gov/pubmed/21164738 http://dx.doi.org/10.1364/OE.18.023924 |
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author | Churnside, Allison B. King, Gavin M. Perkins, Thomas T. |
author_facet | Churnside, Allison B. King, Gavin M. Perkins, Thomas T. |
author_sort | Churnside, Allison B. |
collection | PubMed |
description | In atomic force microscopy (AFM), finding sparsely distributed regions of interest can be difficult and time-consuming. Typically, the tip is scanned until the desired object is located. This process can mechanically or chemically degrade the tip, as well as damage fragile biological samples. Protein assemblies can be detected using the back-scattered light from a focused laser beam. We previously used back-scattered light from a pair of laser foci to stabilize an AFM. In the present work, we integrate these techniques to optically image patches of purple membranes prior to AFM investigation. These rapidly acquired optical images were aligned to the subsequent AFM images to ~40 nm, since the tip position was aligned to the optical axis of the imaging laser. Thus, this label-free imaging efficiently locates sparsely distributed protein assemblies for subsequent AFM study while simultaneously minimizing degradation of the tip and the sample. |
format | Online Article Text |
id | pubmed-3158999 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-31589992011-08-21 Label-free optical imaging of membrane patches for atomic force microscopy Churnside, Allison B. King, Gavin M. Perkins, Thomas T. Opt Express Research-Article In atomic force microscopy (AFM), finding sparsely distributed regions of interest can be difficult and time-consuming. Typically, the tip is scanned until the desired object is located. This process can mechanically or chemically degrade the tip, as well as damage fragile biological samples. Protein assemblies can be detected using the back-scattered light from a focused laser beam. We previously used back-scattered light from a pair of laser foci to stabilize an AFM. In the present work, we integrate these techniques to optically image patches of purple membranes prior to AFM investigation. These rapidly acquired optical images were aligned to the subsequent AFM images to ~40 nm, since the tip position was aligned to the optical axis of the imaging laser. Thus, this label-free imaging efficiently locates sparsely distributed protein assemblies for subsequent AFM study while simultaneously minimizing degradation of the tip and the sample. Optical Society of America 2010-10-29 /pmc/articles/PMC3158999/ /pubmed/21164738 http://dx.doi.org/10.1364/OE.18.023924 Text en ©2010 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Research-Article Churnside, Allison B. King, Gavin M. Perkins, Thomas T. Label-free optical imaging of membrane patches for atomic force microscopy |
title | Label-free optical imaging of membrane patches for atomic force microscopy |
title_full | Label-free optical imaging of membrane patches for atomic force microscopy |
title_fullStr | Label-free optical imaging of membrane patches for atomic force microscopy |
title_full_unstemmed | Label-free optical imaging of membrane patches for atomic force microscopy |
title_short | Label-free optical imaging of membrane patches for atomic force microscopy |
title_sort | label-free optical imaging of membrane patches for atomic force microscopy |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158999/ https://www.ncbi.nlm.nih.gov/pubmed/21164738 http://dx.doi.org/10.1364/OE.18.023924 |
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