Cargando…
High-level transgene expression by homologous recombination-mediated gene transfer
Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of...
Autores principales: | Grandjean, Mélanie, Girod, Pierre-Alain, Calabrese, David, Kostyrko, Kaja, Wicht, Marianne, Yerly, Florence, Mazza, Christian, Beckmann, Jacques S., Martinet, Danielle, Mermod, Nicolas |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159483/ https://www.ncbi.nlm.nih.gov/pubmed/21652640 http://dx.doi.org/10.1093/nar/gkr436 |
Ejemplares similares
-
Assays for DNA double-strand break repair by microhomology-based end-joining repair mechanisms
por: Kostyrko, Kaja, et al.
Publicado: (2016) -
Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA
por: Langerak, Petra, et al.
Publicado: (2005) -
Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM
por: Finney-Manchester, Shawn P., et al.
Publicado: (2013) -
Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
por: Chen, Changchun, et al.
Publicado: (2013) -
MAR‐Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering
por: Kostyrko, Kaja, et al.
Publicado: (2016)