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Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

In utero survival surgery in mice permits the molecular manipulation of gene expression during development. However, because the uterine wall is opaque during early embryogenesis, the ability to target specific parts of the embryo for microinjection is greatly limited. Fortunately, high-frequency ul...

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Detalles Bibliográficos
Autores principales: Pierfelice, Tarran J., Gaiano, Nicholas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159583/
https://www.ncbi.nlm.nih.gov/pubmed/21113114
http://dx.doi.org/10.3791/2047