Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures

In this study, we outline a standardized protocol for the successful cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. For this protocol the freezing medium used is 10% dimethyl sulfoxide (DMSO) diluted in Hank's Buffered Salt Solution (...

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Detalles Bibliográficos
Autores principales: Rahman, Ardeshir S., Parvinjah, Shaudee, Hanna, Michael A., Helguera, Pablo R., Busciglio, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2010
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159608/
https://www.ncbi.nlm.nih.gov/pubmed/21113113
http://dx.doi.org/10.3791/2384

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159608/
https://www.ncbi.nlm.nih.gov/pubmed/21113113
http://dx.doi.org/10.3791/2384

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