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Reverse Transcriptase-Coupled Quantitative Real Time PCR Analysis of Cell-Free Transcription on the Chromatin-Assembled p21 Promoter

BACKGROUND: Cell-free eukaryotic transcription assays have contributed tremendously to the current understanding of the molecular mechanisms that govern transcription at eukaryotic promoters. Currently, the conventional G-less cassette transcription assay is one of the simplest and fastest methods f...

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Detalles Bibliográficos
Autores principales: Park, Jeong Hyeon, Magan, Natisha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3160311/
https://www.ncbi.nlm.nih.gov/pubmed/21886803
http://dx.doi.org/10.1371/journal.pone.0023617