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Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70

BACKGROUND: Escherichia coli cell-free expression systems use bacteriophage RNA polymerases, such as T7, to synthesize large amounts of recombinant proteins. These systems are used for many applications in biotechnology, such as proteomics. Recently, informational processes have been reconstituted i...

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Detalles Bibliográficos
Autores principales: Shin, Jonghyeon, Noireaux, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161345/
https://www.ncbi.nlm.nih.gov/pubmed/20576148
http://dx.doi.org/10.1186/1754-1611-4-8