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Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells
BACKGROUND: The isolation of DNA-free RNA is a crucial step in the reverse transcription PCR (RT-PCR). Every RNA extraction procedure results in RNA samples contaminated with genomic DNA, which can cause false-positive outcomes in highly sensitive applications, including a recently developed quantit...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161365/ https://www.ncbi.nlm.nih.gov/pubmed/20663210 http://dx.doi.org/10.1186/1756-0500-3-211 |