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SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
BACKGROUND: Real-time PCR can be carried out using either probes or DNA dyes. SYBR Green has been used the most, but it suffers from several drawbacks. Numerous other DNA dyes are commercially available, but with limited structural information. Dye behavior in real time PCR is difficult to predict,...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162529/ https://www.ncbi.nlm.nih.gov/pubmed/21798028 http://dx.doi.org/10.1186/1756-0500-4-263 |