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Rapid optimization of gene dosage in E. coli using DIAL strains

BACKGROUND: Engineers frequently vary design parameters to optimize the behaviour of a system. However, synthetic biologists lack the tools to rapidly explore a critical design parameter, gene expression level, and have no means of systematically varying the dosage of an entire genetic circuit. As a...

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Detalles Bibliográficos
Autores principales: Kittleson, Joshua T, Cheung, Sherine, Anderson, J Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163176/
https://www.ncbi.nlm.nih.gov/pubmed/21787416
http://dx.doi.org/10.1186/1754-1611-5-10