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Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: lelpful hints for the optimized nanomagnetisable matrix preparation

BACKGROUND: Several materials are available in the market that work on the principle of protein magnetic fishing by their histidine (His) tags. Little information is available on their performance and it is often quoted that greatly improved purification of histidine-tagged proteins from crude extra...

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Detalles Bibliográficos
Autores principales: Nejadmoghaddam, Mohammad Reza, Chamankhah, Mahmood, Zarei, Saeed, Zarnani, Amir Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163181/
https://www.ncbi.nlm.nih.gov/pubmed/21824404
http://dx.doi.org/10.1186/1477-3155-9-31