Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride

Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived fro...

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Detalles Bibliográficos
Autores principales: Srinivasan, Viswanathan, Sivaramakrishnan, Hariharan, Karthikeyan, Balakrishnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Österreichische Apotheker-Verlagsgesellschaft 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163365/
https://www.ncbi.nlm.nih.gov/pubmed/21886903
http://dx.doi.org/10.3797/scipharm.1101-19
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author Srinivasan, Viswanathan
Sivaramakrishnan, Hariharan
Karthikeyan, Balakrishnan
author_facet Srinivasan, Viswanathan
Sivaramakrishnan, Hariharan
Karthikeyan, Balakrishnan
author_sort Srinivasan, Viswanathan
collection PubMed
description Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived from two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and mass spectrometry (MS), impurity-1 and impurity-2 were characterized as 2-(3,4-dimethoxyphenyl)-3-methylbut-2-enenitrile and 2-(3,4-dimethoxyphenyl)-2-isopropyl-3-methylbutanenitrile, respectively.
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spelling pubmed-31633652011-09-01 Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride Srinivasan, Viswanathan Sivaramakrishnan, Hariharan Karthikeyan, Balakrishnan Sci Pharm Research Article Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived from two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and mass spectrometry (MS), impurity-1 and impurity-2 were characterized as 2-(3,4-dimethoxyphenyl)-3-methylbut-2-enenitrile and 2-(3,4-dimethoxyphenyl)-2-isopropyl-3-methylbutanenitrile, respectively. Österreichische Apotheker-Verlagsgesellschaft 2011 2011-05-08 /pmc/articles/PMC3163365/ /pubmed/21886903 http://dx.doi.org/10.3797/scipharm.1101-19 Text en © Srinivasan et al.; licensee Österreichische Apotheker-Verlagsgesellschaft m. b. H., Vienna, Austria. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Srinivasan, Viswanathan
Sivaramakrishnan, Hariharan
Karthikeyan, Balakrishnan
Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title_full Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title_fullStr Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title_full_unstemmed Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title_short Detection, Isolation and Characterization of Principal Synthetic Route Indicative Impurities in Verapamil Hydrochloride
title_sort detection, isolation and characterization of principal synthetic route indicative impurities in verapamil hydrochloride
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163365/
https://www.ncbi.nlm.nih.gov/pubmed/21886903
http://dx.doi.org/10.3797/scipharm.1101-19
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