Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire

BACKGROUND: Tanganya virus (TGNV), the only shrew-associated hantavirus reported to date from sub-Saharan Africa, is harbored by the Therese's shrew (Crocidura theresae), and is phylogenetically distinct from Thottapalayam virus (TPMV) in the Asian house shrew (Suncus murinus) and Imjin virus (...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Hae Ji, Kadjo, Blaise, Dubey, Sylvain, Jacquet, François, Yanagihara, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163557/
https://www.ncbi.nlm.nih.gov/pubmed/21798050
http://dx.doi.org/10.1186/1743-422X-8-373
_version_ 1782210960882139136
author Kang, Hae Ji
Kadjo, Blaise
Dubey, Sylvain
Jacquet, François
Yanagihara, Richard
author_facet Kang, Hae Ji
Kadjo, Blaise
Dubey, Sylvain
Jacquet, François
Yanagihara, Richard
author_sort Kang, Hae Ji
collection PubMed
description BACKGROUND: Tanganya virus (TGNV), the only shrew-associated hantavirus reported to date from sub-Saharan Africa, is harbored by the Therese's shrew (Crocidura theresae), and is phylogenetically distinct from Thottapalayam virus (TPMV) in the Asian house shrew (Suncus murinus) and Imjin virus (MJNV) in the Ussuri white-toothed shrew (Crocidura lasiura). The existence of myriad soricid-borne hantaviruses in Eurasia and North America would predict the presence of additional hantaviruses in sub-Saharan Africa, where multiple shrew lineages have evolved and diversified. METHODS: Lung tissues, collected in RNAlater(®), from 39 Buettikofer's shrews (Crocidura buettikoferi), 5 Jouvenet's shrews (Crocidura jouvenetae), 9 West African pygmy shrews (Crocidura obscurior) and 21 African giant shrews (Crocidura olivieri) captured in Côte d'Ivoire during 2009, were systematically examined for hantavirus RNA by RT-PCR. RESULTS: A genetically distinct hantavirus, designated Azagny virus (AZGV), was detected in the West African pygmy shrew. Phylogenetic analysis of the S, M and L segments, using maximum-likelihood and Bayesian methods, under the GTR+I+Γ model of evolution, showed that AZGV shared a common ancestry with TGNV and was more closely related to hantaviruses harbored by soricine shrews than to TPMV and MJNV. That is, AZGV in the West African pygmy shrew, like TGNV in the Therese's shrew, did not form a monophyletic group with TPMV and MJNV, which were deeply divergent and basal to other rodent- and soricomorph-borne hantaviruses. Ancestral distributions of each hantavirus lineage, reconstructed using Mesquite 2.74, suggested that the common ancestor of all hantaviruses was most likely of Eurasian, not African, origin. CONCLUSIONS: Genome-wide analysis of many more hantaviruses from sub-Saharan Africa are required to better understand how the biogeographic origin and radiation of African shrews might have contributed to, or have resulted from, the evolution of hantaviruses.
format Online
Article
Text
id pubmed-3163557
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-31635572011-08-30 Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire Kang, Hae Ji Kadjo, Blaise Dubey, Sylvain Jacquet, François Yanagihara, Richard Virol J Research BACKGROUND: Tanganya virus (TGNV), the only shrew-associated hantavirus reported to date from sub-Saharan Africa, is harbored by the Therese's shrew (Crocidura theresae), and is phylogenetically distinct from Thottapalayam virus (TPMV) in the Asian house shrew (Suncus murinus) and Imjin virus (MJNV) in the Ussuri white-toothed shrew (Crocidura lasiura). The existence of myriad soricid-borne hantaviruses in Eurasia and North America would predict the presence of additional hantaviruses in sub-Saharan Africa, where multiple shrew lineages have evolved and diversified. METHODS: Lung tissues, collected in RNAlater(®), from 39 Buettikofer's shrews (Crocidura buettikoferi), 5 Jouvenet's shrews (Crocidura jouvenetae), 9 West African pygmy shrews (Crocidura obscurior) and 21 African giant shrews (Crocidura olivieri) captured in Côte d'Ivoire during 2009, were systematically examined for hantavirus RNA by RT-PCR. RESULTS: A genetically distinct hantavirus, designated Azagny virus (AZGV), was detected in the West African pygmy shrew. Phylogenetic analysis of the S, M and L segments, using maximum-likelihood and Bayesian methods, under the GTR+I+Γ model of evolution, showed that AZGV shared a common ancestry with TGNV and was more closely related to hantaviruses harbored by soricine shrews than to TPMV and MJNV. That is, AZGV in the West African pygmy shrew, like TGNV in the Therese's shrew, did not form a monophyletic group with TPMV and MJNV, which were deeply divergent and basal to other rodent- and soricomorph-borne hantaviruses. Ancestral distributions of each hantavirus lineage, reconstructed using Mesquite 2.74, suggested that the common ancestor of all hantaviruses was most likely of Eurasian, not African, origin. CONCLUSIONS: Genome-wide analysis of many more hantaviruses from sub-Saharan Africa are required to better understand how the biogeographic origin and radiation of African shrews might have contributed to, or have resulted from, the evolution of hantaviruses. BioMed Central 2011-07-28 /pmc/articles/PMC3163557/ /pubmed/21798050 http://dx.doi.org/10.1186/1743-422X-8-373 Text en Copyright ©2011 Kang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kang, Hae Ji
Kadjo, Blaise
Dubey, Sylvain
Jacquet, François
Yanagihara, Richard
Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title_full Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title_fullStr Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title_full_unstemmed Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title_short Molecular evolution of Azagny virus, a newfound hantavirus harbored by the West African pygmy shrew (Crocidura obscurior) in Côte d'Ivoire
title_sort molecular evolution of azagny virus, a newfound hantavirus harbored by the west african pygmy shrew (crocidura obscurior) in côte d'ivoire
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3163557/
https://www.ncbi.nlm.nih.gov/pubmed/21798050
http://dx.doi.org/10.1186/1743-422X-8-373
work_keys_str_mv AT kanghaeji molecularevolutionofazagnyvirusanewfoundhantavirusharboredbythewestafricanpygmyshrewcrociduraobscuriorincotedivoire
AT kadjoblaise molecularevolutionofazagnyvirusanewfoundhantavirusharboredbythewestafricanpygmyshrewcrociduraobscuriorincotedivoire
AT dubeysylvain molecularevolutionofazagnyvirusanewfoundhantavirusharboredbythewestafricanpygmyshrewcrociduraobscuriorincotedivoire
AT jacquetfrancois molecularevolutionofazagnyvirusanewfoundhantavirusharboredbythewestafricanpygmyshrewcrociduraobscuriorincotedivoire
AT yanagihararichard molecularevolutionofazagnyvirusanewfoundhantavirusharboredbythewestafricanpygmyshrewcrociduraobscuriorincotedivoire