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Get Phases from Arsenic Anomalous Scattering: de novo SAD Phasing of Two Protein Structures Crystallized in Cacodylate Buffer

The crystal structures of two proteins, a putative pyrazinamidase/nicotinamidase from the dental pathogen Streptococcus mutans (SmPncA) and the human caspase-6 (Casp6), were solved by de novo arsenic single-wavelength anomalous diffraction (As-SAD) phasing method. Arsenic (As), an uncommonly used el...

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Detalles Bibliográficos
Autores principales: Liu, Xiang, Zhang, Heng, Wang, Xiao-Jun, Li, Lan-Fen, Su, Xiao-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3166297/
https://www.ncbi.nlm.nih.gov/pubmed/21912678
http://dx.doi.org/10.1371/journal.pone.0024227