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Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction

A comparison of histochemical detection of GM1 ganglioside in cryostat sections using cholera toxin B-subunit after fixation with 4% formaldehyde and dry acetone gave tissue-dependent results. In the liver no pre-treatment showed detectable differences related to GM1 reaction products, while studies...

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Detalles Bibliográficos
Autores principales: Petr, T., Šmíd, V., Šmídová, J., Hůlková, H., Jirkovská, M., Elleder, M., Muchová, L., Vítek, L., Šmíd, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167299/
https://www.ncbi.nlm.nih.gov/pubmed/20558344
http://dx.doi.org/10.4081/ejh.2010.e23