Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors

Insertion of a transgene into a defined genomic locus in human embryonic stem cells (hESCs) is crucial in preventing random integration-induced insertional mutagenesis, and can possibly enable persistent transgene expression during hESC expansion and in their differentiated progenies. Here, we emplo...

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Detalles Bibliográficos
Autores principales: Ramachandra, Chrishan J. A., Shahbazi, Mohammad, Kwang, Timothy W. X., Choudhury, Yukti, Bak, Xiao Ying, Yang, Jing, Wang, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167641/
https://www.ncbi.nlm.nih.gov/pubmed/21685448
http://dx.doi.org/10.1093/nar/gkr409

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167641/
https://www.ncbi.nlm.nih.gov/pubmed/21685448
http://dx.doi.org/10.1093/nar/gkr409

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