Characterization of polybacterial clinical samples using a set of group-specific broad-range primers targeting the 16S rRNA gene followed by DNA sequencing and RipSeq analysis

The standard use of a single universal broad-range PCR in direct 16S rDNA sequencing from polybacterial samples leaves the minor constituents at risk of remaining undetected because all bacterial DNA will be competing for the same reagents. In this article we introduce a set of three broad-range gro...

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Detalles Bibliográficos
Autores principales: Kommedal, Øyvind, Lekang, Katrine, Langeland, Nina, Wiker, Harald G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2011
Materias:
Diagnostics, Typing and Identification
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168215/
https://www.ncbi.nlm.nih.gov/pubmed/21436365
http://dx.doi.org/10.1099/jmm.0.028373-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168215/
https://www.ncbi.nlm.nih.gov/pubmed/21436365
http://dx.doi.org/10.1099/jmm.0.028373-0

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