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Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the l...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168274/ https://www.ncbi.nlm.nih.gov/pubmed/21912736 http://dx.doi.org/10.4061/2011/572873 |
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author | Kobayashi, Keita Matsui, Noriko Usui, Kenji |
author_facet | Kobayashi, Keita Matsui, Noriko Usui, Kenji |
author_sort | Kobayashi, Keita |
collection | PubMed |
description | We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. |
format | Online Article Text |
id | pubmed-3168274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-31682742011-09-12 Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence Kobayashi, Keita Matsui, Noriko Usui, Kenji J Nucleic Acids Research Article We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. SAGE-Hindawi Access to Research 2011 2011-09-06 /pmc/articles/PMC3168274/ /pubmed/21912736 http://dx.doi.org/10.4061/2011/572873 Text en Copyright © 2011 Keita Kobayashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kobayashi, Keita Matsui, Noriko Usui, Kenji Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title | Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title_full | Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title_fullStr | Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title_full_unstemmed | Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title_short | Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence |
title_sort | use of a designed peptide library to screen for binders to a particular dna g-quadruplex sequence |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168274/ https://www.ncbi.nlm.nih.gov/pubmed/21912736 http://dx.doi.org/10.4061/2011/572873 |
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