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Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence

We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the l...

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Detalles Bibliográficos
Autores principales: Kobayashi, Keita, Matsui, Noriko, Usui, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168274/
https://www.ncbi.nlm.nih.gov/pubmed/21912736
http://dx.doi.org/10.4061/2011/572873
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author Kobayashi, Keita
Matsui, Noriko
Usui, Kenji
author_facet Kobayashi, Keita
Matsui, Noriko
Usui, Kenji
author_sort Kobayashi, Keita
collection PubMed
description We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering.
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spelling pubmed-31682742011-09-12 Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence Kobayashi, Keita Matsui, Noriko Usui, Kenji J Nucleic Acids Research Article We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. SAGE-Hindawi Access to Research 2011 2011-09-06 /pmc/articles/PMC3168274/ /pubmed/21912736 http://dx.doi.org/10.4061/2011/572873 Text en Copyright © 2011 Keita Kobayashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kobayashi, Keita
Matsui, Noriko
Usui, Kenji
Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title_full Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title_fullStr Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title_full_unstemmed Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title_short Use of a Designed Peptide Library to Screen for Binders to a Particular DNA G-Quadruplex Sequence
title_sort use of a designed peptide library to screen for binders to a particular dna g-quadruplex sequence
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168274/
https://www.ncbi.nlm.nih.gov/pubmed/21912736
http://dx.doi.org/10.4061/2011/572873
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