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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site

Yeast two-hybrid (Y2H) and isothermal titration calorimetry (ITC) methods were used to further study the mutational effect of non-erythroid alpha spectrin (αII) at position 22 in tetramer formation with beta spectrin (βII). Four mutants, αII-V22D, V22F, V22M and V22W, were studied. For the Y2H syste...

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Detalles Bibliográficos
Autores principales: Sevinc, Akin, Witek, Marta A., Fung, Leslie W. -M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SP Versita 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169182/
https://www.ncbi.nlm.nih.gov/pubmed/21786033
http://dx.doi.org/10.2478/s11658-011-0017-9