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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation(1). Due to its capacity to undergo efficient mitotic homologous recombination...

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Detalles Bibliográficos
Autores principales: Liu, Yen-Chun, Vidali, Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169274/
https://www.ncbi.nlm.nih.gov/pubmed/21540817
http://dx.doi.org/10.3791/2560