Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SS...

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Detalles Bibliográficos
Autores principales: Choi, Ryan, Kelley, Angela, Leibly, David, Nakazawa Hewitt, Stephen, Napuli, Alberto, Van Voorhis, Wesley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Laboratory Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169392/
https://www.ncbi.nlm.nih.gov/pubmed/21904040
http://dx.doi.org/10.1107/S1744309111017374

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169392/
https://www.ncbi.nlm.nih.gov/pubmed/21904040
http://dx.doi.org/10.1107/S1744309111017374

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