Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline

Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. The SSGCID program uses a wheat germ...

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Detalles Bibliográficos
Autores principales: Guild, Katherine, Zhang, Yang, Stacy, Robin, Mundt, Elizabeth, Benbow, Sarah, Green, Amanda, Myler, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Laboratory Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169397/
https://www.ncbi.nlm.nih.gov/pubmed/21904045
http://dx.doi.org/10.1107/S1744309111032143

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169397/
https://www.ncbi.nlm.nih.gov/pubmed/21904045
http://dx.doi.org/10.1107/S1744309111032143

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