Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

INTRODUCTION: The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis. METHODS: We present a method consisting of electrophoresis of complex...

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Detalles Bibliográficos
Autores principales: Seshi, Beerelli, Raja, Kumaraguru, Chandramouli, KH
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170271/
https://www.ncbi.nlm.nih.gov/pubmed/21906381
http://dx.doi.org/10.1186/1559-0275-8-10

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170271/
https://www.ncbi.nlm.nih.gov/pubmed/21906381
http://dx.doi.org/10.1186/1559-0275-8-10

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