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Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis
INTRODUCTION: The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis. METHODS: We present a method consisting of electrophoresis of complex...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170271/ https://www.ncbi.nlm.nih.gov/pubmed/21906381 http://dx.doi.org/10.1186/1559-0275-8-10 |